› 3fqa

X-ray diffraction
2.35Å resolution

Gabaculien complex of gabaculine resistant GSAM version

Released:
DOI: 10.2210/pdb3fqa/pdb
PDB entry 3fqa coloured by chain, front view.
PDB entry 3fqa coloured by chain, side view.
PDB entry 3fqa coloured by chain, top view.
Source organism: Synechococcus elongatus PCC 6301
Primary publication:
Absence of a catalytic water confers resistance to the neurotoxin gabaculine.
Orriss GL, Patel TR, Sorensen J, Stetefeld J
FASEB J 24 404-14 (2010)
PMID: 19786580

Function and Biology Details

Reaction catalysed: 
(S)-4-amino-5-oxopentanoate = 5-aminolevulinate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150263 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate-1-semialdehyde 2,1-aminomutase Chains: A, B
Molecule details ›
Chains: A, B
Length: 427 amino acids
Theoretical weight: 45.57 KDa
Source organism: Synechococcus elongatus PCC 6301
Expression system: Escherichia coli
UniProt:
  • Canonical: P24630 (Residues: 7-433; Coverage: 99%)
Gene names: gsa, hemL, syc0881_c
Sequence domains: Aminotransferase class-III
Structure domains:

Ligands and Environments


Cofactor: Ligand PMP 2 x PMP
1 bound ligand:
Ligand GAB 1 x GAB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 67.938Å b: 107.868Å c: 123.066Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.208 0.241
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Structure and function of enzymes in heme biosynthesis.
Layer et al. (2010)
1 more

1 mention without citation

Modulation of mitochondrial activity by sugarcane (Saccharum officinarum L.) top extract and its bioactive polyphenols: a comprehensive transcriptomics analysis in C2C12 myotubes and HepG2 hepatocytes.
Iwata et al. (2024)