› 3fqg

X-ray diffraction
2Å resolution

Crystal Structure of the S. pombe Rai1

Released:
DOI: 10.2210/pdb3fqg/pdb
PDB entry 3fqg coloured by chain, front view.
PDB entry 3fqg coloured by chain, side view.
PDB entry 3fqg coloured by chain, top view.
Source organism: Schizosaccharomyces pombe
Primary publication:
Structure and function of the 5'-->3' exoribonuclease Rat1 and its activating partner Rai1.
Xiang S, Cooper-Morgan A, Jiao X, Kiledjian M, Manley JL, Tong L
Nature 458 784-8 (2009)
PMID: 19194460

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126921 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Decapping nuclease din1 Chain: A
Molecule details ›
Chain: A
Length: 360 amino acids
Theoretical weight: 41.87 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Escherichia coli
UniProt:
  • Canonical: O13836 (Residues: 1-352; Coverage: 100%)
Gene names: SPAC19D5.06c, din1, rai1
Sequence domains: RAI1 like PD-(D/E)XK nuclease

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: C2
Unit cell:
a: 101.597Å b: 60.79Å c: 73.124Å
α: 90° β: 103.01° γ: 90°
R-values:
R R work R free
0.197 0.195 0.23
Expression system: Escherichia coli

Quick links

Citations

42 review citations

RNA degradation in Saccharomyces cerevisae.
Parker R. (2012)
41 more

2 mentions without citation

Structure and function of pre-mRNA 5'-end capping quality control and 3'-end processing.
Jurado et al. (2014)
1 more