› 3frx

X-ray diffraction
2.13Å resolution

Crystal Structure of the Yeast Orthologue of RACK1, Asc1.

Released:
DOI: 10.2210/pdb3frx/pdb
PDB entry 3frx coloured by chain, front view.
PDB entry 3frx coloured by chain, side view.
PDB entry 3frx coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Direct link between RACK1 function and localization at the ribosome in vivo.
Coyle SM, Gilbert WV, Doudna JA
Mol Cell Biol 29 1626-34 (2009)
PMID: 19114558

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153528 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Small ribosomal subunit protein RACK1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 319 amino acids
Theoretical weight: 35.17 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P38011 (Residues: 1-319; Coverage: 100%)
Gene names: ASC1, CPC2, YM9718.15C, YMR116C
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase

Ligands and Environments

1 bound ligand:
Ligand MN 4 x MN
1 modified residue:
Ligand MSE 28 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: C2
Unit cell:
a: 168.66Å b: 85.41Å c: 119.1Å
α: 90° β: 134.8° γ: 90°
R-values:
R R work R free
0.196 0.195 0.239
Expression system: Escherichia coli

Quick links

Citations

19 review citations

How common are extraribosomal functions of ribosomal proteins?
Warner et al. (2009)
18 more

11 mentions without citation

RACK1, A multifaceted scaffolding protein: Structure and function.
Adams et al. (2011)
10 more