› 3fs0

X-ray diffraction
2.3Å resolution

Class II ligase ribozyme product-template duplex, structure 2

Released:
DOI: 10.2210/pdb3fs0/pdb
PDB entry 3fs0 coloured by chain, front view.
PDB entry 3fs0 coloured by chain, side view.
PDB entry 3fs0 coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Structure-guided engineering of the regioselectivity of RNA ligase ribozymes.
Pitt JN, Ferré-D'Amaré AR
J Am Chem Soc 131 3532-40 (2009)
PMID: 19220054

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196672 (preferred)
Entry contents:
2 distinct RNA molecules
Macromolecules (2 distinct):
5'-R(*GP*GP*UP*GP*AP*GP*GP*CP*UP*G)-3' Chain: A
Molecule details ›
Chain: A
Length: 10 nucleotides
Theoretical weight: 3.27 KDa
Source organism: Synthetic construct
Expression system: Not provided
5'-R(*CP*CP*AP*GP*UP*CP*GP*GP*AP*AP*C)-3' Chain: B
Molecule details ›
Chain: B
Length: 11 nucleotides
Theoretical weight: 3.51 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 5 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P31
Unit cell:
a: 30.04Å b: 30.04Å c: 59.666Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.208 0.208 0.234
Expression system: Not provided

Quick links

Citations

4 review citations

Ribozymes and riboswitches: modulation of RNA function by small molecules.
Zhang et al. (2010)
3 more

4 mentions without citation

Sequence dependent variations in RNA duplex are related to non-canonical hydrogen bond interactions in dinucleotide steps.
Kailasam et al. (2014)
3 more