› 3ftg

X-ray diffraction
2.6Å resolution

Crystal Structure of H2Db in complex with NP366-N3A variant peptide from influenza

Released:
DOI: 10.2210/pdb3ftg/pdb
PDB entry 3ftg coloured by chain, front view.
PDB entry 3ftg coloured by chain, side view.
PDB entry 3ftg coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Protective efficacy of cross-reactive CD8+ T cells recognising mutant viral epitopes depends on peptide-MHC-I structural interactions and T cell activation threshold.
Valkenburg SA, Gras S, Guillonneau C, La Gruta NL, Thomas PG, Purcell AW, Rossjohn J, Doherty PC, Turner SJ, Kedzierska K
PLoS Pathog 6 e1001039 (2010)
PMID: 20711359

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-135004 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
H-2 class I histocompatibility antigen, D-B alpha chain Chain: A
Molecule details ›
Chain: A
Length: 281 amino acids
Theoretical weight: 32.6 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01899 (Residues: 25-304; Coverage: 83%)
Gene name: H2-D1
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.84 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01887 (Residues: 21-119; Coverage: 100%)
Gene name: B2m
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
NP366-N3A variant peptide from influenza virus Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 983 Da

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: I222
Unit cell:
a: 93.64Å b: 94.98Å c: 132.24Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.221 0.304
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Bias in the αβ T-cell repertoire: implications for disease pathogenesis and vaccination.
Miles et al. (2011)
9 more

3 mentions without citation

Protective efficacy of cross-reactive CD8+ T cells recognising mutant viral epitopes depends on peptide-MHC-I structural interactions and T cell activation threshold.
Valkenburg et al. (2010)
2 more