› 3ftm

X-ray diffraction
2.7Å resolution

Class II ligase ribozyme product-template duplex, structure 1

Released:
DOI: 10.2210/pdb3ftm/pdb
PDB entry 3ftm coloured by chain, front view.
PDB entry 3ftm coloured by chain, side view.
PDB entry 3ftm coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Structure-guided engineering of the regioselectivity of RNA ligase ribozymes.
Pitt JN, Ferré-D'Amaré AR
J Am Chem Soc 131 3532-40 (2009)
PMID: 19220054

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196673 (preferred)
Entry contents:
2 distinct RNA molecules
Macromolecules (2 distinct):
5'-R(*GP*UP*GP*UP*GP*AP*GP*GP*CP*UP*G)-3' Chains: A, C
Molecule details ›
Chains: A, C
Length: 11 nucleotides
Theoretical weight: 3.58 KDa
Source organism: Synthetic construct
Expression system: Not provided
5'-R(*CP*CP*AP*GP*UP*CP*GP*GP*AP*AP*CP*A)-3' Chains: B, D
Molecule details ›
Chains: B, D
Length: 12 nucleotides
Theoretical weight: 3.83 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MG 9 x MG
Ligand K 2 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P41
Unit cell:
a: 31.55Å b: 31.55Å c: 171.066Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.218 0.268
Expression system: Not provided

Quick links

Citations

4 review citations

Ribozymes and riboswitches: modulation of RNA function by small molecules.
Zhang et al. (2010)
3 more

1 mention without citation

Sequence dependent variations in RNA duplex are related to non-canonical hydrogen bond interactions in dinucleotide steps.
Kailasam et al. (2014)