› 3fu2

X-ray diffraction
2.85Å resolution

Cocrystal structure of a class-I preQ1 riboswitch

Released:
DOI: 10.2210/pdb3fu2/pdb
PDB entry 3fu2 coloured by chain, front view.
PDB entry 3fu2 coloured by chain, side view.
PDB entry 3fu2 coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Cocrystal structure of a class I preQ1 riboswitch reveals a pseudoknot recognizing an essential hypermodified nucleobase.
Klein DJ, Edwards TE, Ferré-D'Amaré AR
Nat Struct Mol Biol 16 343-4 (2009)
PMID: 19234468

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196674 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
PreQ1 riboswitch Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 34 nucleotides
Theoretical weight: 10.84 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand PRF 3 x PRF
Ligand CA 9 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P3221
Unit cell:
a: 78.208Å b: 78.208Å c: 85.22Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.214 0.214 0.267
Expression system: Not provided

Quick links

Citations

21 review citations

Riboswitches: discovery of drugs that target bacterial gene-regulatory RNAs.
Deigan et al. (2011)
20 more

20 mentions without citation

Metabolite recognition principles and molecular mechanisms underlying riboswitch function.
Serganov et al. (2012)
19 more