› 3fu8

X-ray diffraction
1.8Å resolution

Melanocarpus albomyces laccase crystal soaked (10 sec) with 2,6-dimethoxyphenol

Released:
DOI: 10.2210/pdb3fu8/pdb
PDB entry 3fu8 coloured by chain, front view.
PDB entry 3fu8 coloured by chain, side view.
PDB entry 3fu8 coloured by chain, top view.
Source organism: Melanocarpus albomyces
Primary publication:
Structure-function studies of a Melanocarpus albomyces laccase suggest a pathway for oxidation of phenolic compounds.
Kallio JP, Auer S, Jänis J, Andberg M, Kruus K, Rouvinen J, Koivula A, Hakulinen N
J Mol Biol 392 895-909 (2009)
PMID: 19563811

Function and Biology Details

Reaction catalysed: 
4 benzenediol + O(2) = 4 benzosemiquinone + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180696 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
Laccase-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 559 amino acids
Theoretical weight: 61.86 KDa
Source organism: Melanocarpus albomyces
Expression system: Trichoderma reesei
UniProt:
  • Canonical: Q70KY3 (Residues: 51-609; Coverage: 93%)
Gene name: LAC1
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, MAN
Carbohydrate polymer
6 bound ligands:
Ligand CU 8 x CU
Ligand CL 2 x CL
Ligand NAG 5 x NAG
Ligand SO4 3 x SO4
Ligand 3DM 2 x 3DM
Ligand GOL 2 x GOL
1 modified residue:
Ligand OHI 2 x OHI

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: C2
Unit cell:
a: 174.71Å b: 62.37Å c: 125.14Å
α: 90° β: 96.36° γ: 90°
R-values:
R R work R free
0.206 0.203 0.256
Expression system: Trichoderma reesei

Quick links

Citations

13 review citations

Copper active sites in biology.
Solomon et al. (2014)
12 more

2 mentions without citation

Structure of laccase from Streptomyces coelicolor after soaking with potassium hexacyanoferrate and at an improved resolution of 2.3 Å.
Skálová et al. (2011)
1 more