› 3fwt

X-ray diffraction
1.9Å resolution

Crystal structure of Leishmania major MIF2

Released:
DOI: 10.2210/pdb3fwt/pdb
Model geometry
Fit model/data
PDB entry 3fwt coloured by chain, front view.
PDB entry 3fwt coloured by chain, side view.
PDB entry 3fwt coloured by chain, top view.
Source organism: Leishmania major
Primary publication:
Structures of Leishmania major orthologues of macrophage migration inhibitory factor.
Richardson JM, Morrison LS, Bland ND, Bruce S, Coombs GH, Mottram JC, Walkinshaw MD
Biochem Biophys Res Commun 380 442-8 (2009)
PMID: 19187777

Function and Biology Details

Reactions catalysed: 
Keto-phenylpyruvate = enol-phenylpyruvate
L-dopachrome = 5,6-dihydroxyindole-2-carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175671 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-dopachrome isomerase Chain: A
Molecule details ›
Chain: A
Length: 133 amino acids
Theoretical weight: 14.77 KDa
Source organism: Leishmania major
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4Q412 (Residues: 1-113; Coverage: 100%)
Gene names: LMJF_33_1750, MIF2
Sequence domains: Macrophage migration inhibitory factor (MIF)
Structure domains: Macrophage Migration Inhibitory Factor

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P6322
Unit cell:
a: 53.963Å b: 53.963Å c: 138.902Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.225 0.222 0.28
Expression system: Escherichia coli

Quick links

Citations

3 review citations

The non-mammalian MIF superfamily.
Sparkes et al. (2017)
2 more