› 3fxh

X-ray diffraction
1.84Å resolution

Crystal structure from the mobile metagenome of Halifax Harbour Sewage Outfall: Integron Cassette Protein HFX_CASS2

Released:
DOI: 10.2210/pdb3fxh/pdb
PDB entry 3fxh coloured by chain, front view.
PDB entry 3fxh coloured by chain, side view.
PDB entry 3fxh coloured by chain, top view.
Source organism: uncultured bacterium
Primary publication:
Integron gene cassettes: a repository of novel protein folds with distinct interaction sites.
Sureshan V, Deshpande CN, Boucher Y, Koenig JE, Stokes HW, Harrop SJ, Curmi PM, Mabbutt BC
PLoS One 8 e52934 (2013)
PMID: 23349695

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109115 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative integron gene cassette protein Chain: A
Molecule details ›
Chain: A
Length: 135 amino acids
Theoretical weight: 15.57 KDa
Source organism: uncultured bacterium
Expression system: Escherichia coli
UniProt:
  • Canonical: B0BHE4 (Residues: 10-123; Coverage: 93%)
Gene name: ORF1
Structure domains: Crystal structure from the mobile metagenome of halifax harbour sewage outfall

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: C2
Unit cell:
a: 40.744Å b: 66.829Å c: 48.873Å
α: 90° β: 113.52° γ: 90°
R-values:
R R work R free
0.191 0.186 0.241
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Integrons: past, present, and future.
Gillings MR. (2014)
1 more

1 mention without citation

Integron gene cassettes: a repository of novel protein folds with distinct interaction sites.
Sureshan et al. (2013)