› 3fyx

X-ray diffraction
3.4Å resolution

The Structure of OmpF porin with a synthetic dibenzo-18-crown-6 as modulator

Released:
DOI: 10.2210/pdb3fyx/pdb
PDB entry 3fyx coloured by chain, front view.
PDB entry 3fyx coloured by chain, side view.
PDB entry 3fyx coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
On the function and structure of synthetically modified porins.
Reitz S, Cebi M, Reiss P, Studnik G, Linne U, Koert U, Essen LO
Angew Chem Int Ed Engl 48 4853-7 (2009)
PMID: 19322865

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136399 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Outer membrane porin F Chain: A
Molecule details ›
Chain: A
Length: 340 amino acids
Theoretical weight: 37.09 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P02931 (Residues: 23-362; Coverage: 100%)
Gene names: JW0912, b0929, cmlB, coa, cry, ompF, tolF
Sequence domains: Gram-negative porin
Structure domains: Porin

Ligands and Environments

2 bound ligands:
Ligand 451 1 x 451
Ligand EPE 1 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P321
Unit cell:
a: 135.52Å b: 135.52Å c: 47.52Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.252 0.249 0.294
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Strategies and perspectives in ion-channel engineering.
Grosse et al. (2011)
6 other articles

3 mentions without citation

The structural biology of β-barrel membrane proteins: a summary of recent reports.
Fairman et al. (2011)
2 more