› 3fzy

X-ray diffraction
1.95Å resolution

Crystal Structure of Pre-cleavage Form of Cysteine Protease Domain from Vibrio cholerae RtxA Toxin

Released:
DOI: 10.2210/pdb3fzy/pdb
PDB entry 3fzy coloured by chain, front view.
PDB entry 3fzy coloured by chain, side view.
PDB entry 3fzy coloured by chain, top view.
Source organism: Vibrio cholerae
Primary publication:
Structural and molecular mechanism for autoprocessing of MARTX toxin of Vibrio cholerae at multiple sites.
Prochazkova K, Shuvalova LA, Minasov G, Voburka Z, Anderson WF, Satchell KJ
J Biol Chem 284 26557-68 (2009)
PMID: 19620709

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192064 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ABH effector region toxin F5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 234 amino acids
Theoretical weight: 25.55 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KS12 (Residues: 3440-3650; Coverage: 5%)
Gene names: VC_1451, rtx, rtxA
Sequence domains: Peptidase C80 family
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:
Ligand IHP 2 x IHP
Ligand CL 1 x CL
Ligand UNX 12 x UNX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 46.055Å b: 66.373Å c: 137.958Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.169 0.216
Expression system: Escherichia coli

Quick links

Citations

18 review citations

RTX proteins: a highly diverse family secreted by a common mechanism.
Linhartová et al. (2010)
17 more

7 mentions without citation

Toward a structural understanding of Clostridium difficile toxins A and B.
Pruitt et al. (2012)
6 more