› 3g0t

X-ray diffraction
1.75Å resolution

Crystal structure of putative aspartate aminotransferase (NP_905498.1) from Porphyromonas gingivalis W83 at 1.75 A resolution

Released:
DOI: 10.2210/pdb3g0t/pdb
PDB entry 3g0t coloured by chain, front view.
PDB entry 3g0t coloured by chain, side view.
PDB entry 3g0t coloured by chain, top view.
Source organism: Porphyromonas gingivalis W83
Primary publication:
Molecular characterization of novel pyridoxal-5'-phosphate-dependent enzymes from the human microbiome.
Fleischman NM, Das D, Kumar A, Xu Q, Chiu HJ, Jaroszewski L, Knuth MW, Klock HE, Miller MD, Elsliger MA, Godzik A, Lesley SA, Deacon AM, Wilson IA, Toney MD
Protein Sci 23 1060-76 (2014)
PMID: 24888348

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181749 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminotransferase class I/classII domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 437 amino acids
Theoretical weight: 50.38 KDa
Source organism: Porphyromonas gingivalis W83
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7MUZ3 (Residues: 1-436; Coverage: 100%)
Gene name: PG_1327
Sequence domains: Aminotransferase class I and II
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand NA 2 x NA
Ligand CL 2 x CL
Ligand EDO 19 x EDO
Ligand PE4 2 x PE4
2 modified residues:
Ligand MSE 32 x MSE
Ligand LLP 2 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P21212
Unit cell:
a: 220.44Å b: 54.97Å c: 81.154Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.139 0.137 0.171
Expression system: Escherichia coli

Quick links

Citations

2 review citations

The Impact of Western Diet and Nutrients on the Microbiota and Immune Response at Mucosal Interfaces.
Statovci et al. (2017)
1 more

1 mention without citation

Molecular characterization of novel pyridoxal-5'-phosphate-dependent enzymes from the human microbiome.
Fleischman et al. (2014)