› 3g13

X-ray diffraction
2Å resolution

Crystal structure of putative conjugative transposon recombinase from Clostridium difficile

Released:
DOI: 10.2210/pdb3g13/pdb
PDB entry 3g13 coloured by chain, front view.
PDB entry 3g13 coloured by chain, side view.
PDB entry 3g13 coloured by chain, top view.
Source organism: Clostridioides difficile 630
Entry authors: Bagaria A, Burley SK, Swaminathan S, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172657 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Recombinase Tn916-like, CTn7-Orf1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 169 amino acids
Theoretical weight: 20.14 KDa
Source organism: Clostridioides difficile 630
Expression system: Escherichia coli
UniProt:
  • Canonical: Q180L7 (Residues: 23-180; Coverage: 27%)
Gene name: CD630_33700
Sequence domains: Resolvase, N terminal domain
Structure domains: Resolvase, N-terminal catalytic domain

Ligands and Environments

2 bound ligands:
Ligand GOL 1 x GOL
Ligand NA 1 x NA
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 64.47Å b: 71.318Å c: 94.176Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.199 0.239
Expression system: Escherichia coli

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