› 3g1m

X-ray diffraction
1.7Å resolution

EthR from Mycobacterium tuberculosis in complex with compound BDM31381

Released:
DOI: 10.2210/pdb3g1m/pdb
PDB entry 3g1m coloured by chain, front view.
PDB entry 3g1m coloured by chain, side view.
PDB entry 3g1m coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Synthetic EthR inhibitors boost antituberculous activity of ethionamide.
Willand N, Dirié B, Carette X, Bifani P, Singhal A, Desroses M, Leroux F, Willery E, Mathys V, Déprez-Poulain R, Delcroix G, Frénois F, Aumercier M, Locht C, Villeret V, Déprez B, Baulard AR
Nat Med 15 537-44 (2009)
PMID: 19412174

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161781 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH-type transcriptional regulator EthR Chain: A
Molecule details ›
Chain: A
Length: 236 amino acids
Theoretical weight: 25.95 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WMC1 (Residues: 1-216; Coverage: 100%)
Gene names: Rv3855, etaR, ethR
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand RF3 1 x RF3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P41212
Unit cell:
a: 121.87Å b: 121.87Å c: 33.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.182 0.213
Expression system: Escherichia coli

Quick links

Citations

37 review citations

The TetR family of regulators.
Cuthbertson et al. (2013)
36 more

4 mentions without citation

Structural activation of the transcriptional repressor EthR from Mycobacterium tuberculosis by single amino acid change mimicking natural and synthetic ligands.
Carette et al. (2012)
3 more