› 3g27

X-ray diffraction
2.1Å resolution

Structure of a putative bacteriophage protein from Escherichia coli str. K-12 substr. MG1655

Released:
DOI: 10.2210/pdb3g27/pdb
PDB entry 3g27 coloured by chain, front view.
PDB entry 3g27 coloured by chain, side view.
PDB entry 3g27 coloured by chain, top view.
Source organism: Escherichia coli K-12
Entry authors: Cuff ME, Evdokimova E, Kudritska M, Edwards A, Savchenko A, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159494 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative nuclease YbcO Chain: A
Molecule details ›
Chain: A
Length: 96 amino acids
Theoretical weight: 10.46 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P68661 (Residues: 1-96; Coverage: 100%)
Gene names: JW0537, b0549, ybcO
Sequence domains: Putative nuclease YbcO
Structure domains: prophage-derive protein ybcO

Ligands and Environments

3 bound ligands:
Ligand ZN 1 x ZN
Ligand CA 1 x CA
Ligand EDO 1 x EDO
1 modified residue:
Ligand MSE 2 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P3121
Unit cell:
a: 71.311Å b: 71.311Å c: 32.333Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.188 0.186 0.249
Expression system: Escherichia coli

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