› 3g2q

X-ray diffraction
2.18Å resolution

Crystal Structure of the Glycopeptide N-methyltransferase MtfA complexed with sinefungin

Released:
DOI: 10.2210/pdb3g2q/pdb
PDB entry 3g2q coloured by chain, front view.
PDB entry 3g2q coloured by chain, side view.
PDB entry 3g2q coloured by chain, top view.
Source organism: Amycolatopsis orientalis
Primary publication:
Structure and function of the glycopeptide N-methyltransferase MtfA, a tool for the biosynthesis of modified glycopeptide antibiotics.
Shi R, Lamb SS, Zakeri B, Proteau A, Cui Q, Sulea T, Matte A, Wright GD, Cygler M
Chem Biol 16 401-10 (2009)
PMID: 19389626

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129415 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methyltransferase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 299 amino acids
Theoretical weight: 32.53 KDa
Source organism: Amycolatopsis orientalis
Expression system: Escherichia coli
UniProt:
  • Canonical: O52805 (Residues: 1-280; Coverage: 100%)
Sequence domains: Methyltransferase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SFG 2 x SFG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: C2
Unit cell:
a: 126.611Å b: 71.918Å c: 75.062Å
α: 90° β: 103.82° γ: 90°
R-values:
R R work R free
0.228 0.226 0.253
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Nonribosomal Peptide Synthesis-Principles and Prospects.
Süssmuth et al. (2017)
9 more