› 3g4h

X-ray diffraction
1.92Å resolution

Crystal structure of Human Senescence Marker Protein-30 (Zinc Bound)

Released:
DOI: 10.2210/pdb3g4h/pdb
PDB entry 3g4h coloured by chain, front view.
PDB entry 3g4h coloured by chain, side view.
PDB entry 3g4h coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human senescence marker protein 30: insights linking structural, enzymatic, and physiological functions .
Chakraborti S, Bahnson BJ
Biochemistry 49 3436-44 (2010)
PMID: 20329768

Function and Biology Details

Reaction catalysed: 
D-glucono-1,5-lactone + H(2)O = D-gluconate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172310 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Regucalcin Chains: A, B
Molecule details ›
Chains: A, B
Length: 297 amino acids
Theoretical weight: 33.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15493 (Residues: 3-299; Coverage: 99%)
Gene names: RGN, SMP30
Sequence domains: SMP-30/Gluconolactonase/LRE-like region
Structure domains: TolB, C-terminal domain

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P21
Unit cell:
a: 64.365Å b: 52.019Å c: 85.832Å
α: 90° β: 100.11° γ: 90°
R-values:
R R work R free
0.225 0.224 0.253
Expression system: Escherichia coli

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Citations

9 review citations

Progress in the development of enzyme-based nerve agent bioscavengers.
Nachon et al. (2013)
8 more

3 mentions without citation

Structure and function of aldopentose catabolism enzymes involved in oxidative non-phosphorylative pathways.
Ren et al. (2022)
2 more