› 3g6p

X-ray diffraction
1.98Å resolution

GR DNA binding domain:FKBP5 complex, 18bp

Released:
DOI: 10.2210/pdb3g6p/pdb
PDB entry 3g6p coloured by chain, front view.
PDB entry 3g6p coloured by chain, side view.
PDB entry 3g6p coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
DNA binding site sequence directs glucocorticoid receptor structure and activity.
Meijsing SH, Pufall MA, So AY, Bates DL, Chen L, Yamamoto KR
Science 324 407-10 (2009)
PMID: 19372434

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116269 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Glucocorticoid receptor Chains: A, B
Molecule details ›
Chains: A, B
Length: 90 amino acids
Theoretical weight: 9.96 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P06536 (Residues: 440-525; Coverage: 11%)
Gene names: Grl, Nr3c1
Sequence domains: Zinc finger, C4 type (two domains)
Structure domains: Erythroid Transcription Factor GATA-1, subunit A
DNA (5'-D(*CP*CP*AP*GP*AP*AP*CP*AP*CP*CP*CP*TP*GP*TP*TP*CP*TP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 18 nucleotides
Theoretical weight: 5.44 KDa
Source organism: Rattus norvegicus
Expression system: Not provided
DNA (5'-D(*CP*CP*AP*GP*AP*AP*CP*AP*GP*GP*GP*TP*GP*TP*TP*CP*TP*G)-3') Chain: D
Molecule details ›
Chain: D
Length: 18 nucleotides
Theoretical weight: 5.56 KDa
Source organism: Rattus norvegicus
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand ACT 3 x ACT
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 39.06Å b: 100.11Å c: 112.41Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.214 0.239
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

123 review citations

The anti-inflammatory and immunosuppressive effects of glucocorticoids, recent developments and mechanistic insights.
Coutinho et al. (2011)
122 more

9 mentions without citation

Roles of Prolyl Isomerases in RNA-Mediated Gene Expression.
Thapar R. (2015)
8 more