› 3g77

X-ray diffraction
1.8Å resolution

Bacterial cytosine deaminase V152A/F316C/D317G mutant

Released:
DOI: 10.2210/pdb3g77/pdb
PDB entry 3g77 coloured by chain, front view.
PDB entry 3g77 coloured by chain, side view.
PDB entry 3g77 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Bacterial cytosine deaminase mutants created by molecular engineering show improved 5-fluorocytosine-mediated cell killing in vitro and in vivo.
Fuchita M, Ardiani A, Zhao L, Serve K, Stoddard BL, Black ME
Cancer Res 69 4791-9 (2009)
PMID: 19487291

Function and Biology Details

Reaction catalysed: 
Cytosine + H(2)O = uracil + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150496 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytosine deaminase Chain: A
Molecule details ›
Chain: A
Length: 423 amino acids
Theoretical weight: 47.07 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P25524 (Residues: 5-427; Coverage: 99%)
Gene names: JW0328, b0337, codA
Sequence domains: Amidohydrolase family
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand FE 1 x FE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: R32
Unit cell:
a: 108.938Å b: 108.938Å c: 240.762Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.172 0.171 0.199
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Bystander or no bystander for gene directed enzyme prodrug therapy.
Dachs et al. (2009)
8 more

2 mentions without citation

Bacterial cytosine deaminase mutants created by molecular engineering show improved 5-fluorocytosine-mediated cell killing in vitro and in vivo.
Fuchita et al. (2009)
1 more