› 3g79

X-ray diffraction
2.4Å resolution

Crystal structure of NDP-N-acetyl-D-galactosaminuronic acid dehydrogenase from Methanosarcina mazei Go1

Released:
DOI: 10.2210/pdb3g79/pdb
PDB entry 3g79 coloured by chain, front view.
PDB entry 3g79 coloured by chain, side view.
PDB entry 3g79 coloured by chain, top view.
Source organism: Methanosarcina mazei Go1
Entry authors: Malashkevich VN, Toro R, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
UDP-N-acetyl-alpha-D-mannosamine + 2 NAD(+) + H(2)O = UDP-N-acetyl-alpha-D-mannosaminuronate + 2 NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185821 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-N-acetyl-D-mannosamine dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 478 amino acids
Theoretical weight: 51.98 KDa
Source organism: Methanosarcina mazei Go1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8PXR2 (Residues: 3-469; Coverage: 98%)
Gene name: MM_1154
Sequence domains:
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 78.83Å b: 88.723Å c: 151.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.222 0.296
Expression system: Escherichia coli

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