› 3g7n

X-ray diffraction
1.3Å resolution

Crystal Structure of a Triacylglycerol Lipase from Penicillium Expansum at 1.3

Released:
DOI: 10.2210/pdb3g7n/pdb
PDB entry 3g7n coloured by chain, front view.
PDB entry 3g7n coloured by chain, side view.
PDB entry 3g7n coloured by chain, top view.
Source organism: Penicillium expansum
Primary publication:
Crystal structure of a triacylglycerol lipase from Penicillium expansum at 1.3 A determined by sulfur SAD.
Bian C, Yuan C, Chen L, Meehan EJ, Jiang L, Huang Z, Lin L, Huang M
Proteins 78 1601-5 (2010)
PMID: 20146362

Function and Biology Details

Reaction catalysed: 
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181658 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fungal lipase-like domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 258 amino acids
Theoretical weight: 27.32 KDa
Source organism: Penicillium expansum
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q7LST4 (Residues: 28-285; Coverage: 97%)
Sequence domains: Lipase (class 3)
Structure domains: alpha/beta hydrolase

Ligands and Environments

3 bound ligands:
Ligand SO4 5 x SO4
Ligand PEG 5 x PEG
Ligand 1PE 1 x 1PE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P43212
Unit cell:
a: 88.27Å b: 88.27Å c: 126.616Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.188 0.208
Expression system: Komagataella pastoris

Quick links

Citations

5 citation in other articles

A gene encoding a new cold-active lipase from an Antarctic isolate of Penicillium expansum.
Mohammed et al. (2013)
4 more

2 mentions without citation

Robust structural analysis of native biological macromolecules from multi-crystal anomalous diffraction data.
Liu et al. (2013)
1 more