› 3g84

X-ray diffraction
2.3Å resolution

Crystal structure of the trimeric neck and carbohydrate recognition domain of R343V mutant of human surfactant protein D in complex with alpha 1,2 dimannose.

Released:
DOI: 10.2210/pdb3g84/pdb
PDB entry 3g84 coloured by chain, front view.
PDB entry 3g84 coloured by chain, side view.
PDB entry 3g84 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Recognition of mannosylated ligands and influenza A virus by human surfactant protein D: contributions of an extended site and residue 343.
Crouch E, Hartshorn K, Horlacher T, McDonald B, Smith K, Cafarella T, Seaton B, Seeberger PH, Head J
Biochemistry 48 3335-45 (2009)
PMID: 19249874

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152870 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Pulmonary surfactant-associated protein D Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 160 amino acids
Theoretical weight: 17.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P35247 (Residues: 223-375; Coverage: 43%)
Gene names: COLEC7, PSPD, SFTP4, SFTPD
Sequence domains:
Structure domains: Mannose-Binding Protein A, subunit A

Ligands and Environments

Carbohydrate polymer : NEW Components: MAN
Carbohydrate polymer
1 bound ligand:
Ligand CA 9 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P21
Unit cell:
a: 55.605Å b: 108.066Å c: 55.595Å
α: 90° β: 91.47° γ: 90°
R-values:
R R work R free
0.154 0.152 0.191
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Exploitation of glycosylation in enveloped virus pathobiology.
Watanabe et al. (2019)
9 more

5 mentions without citation

Recognition of mannosylated ligands and influenza A virus by human surfactant protein D: contributions of an extended site and residue 343.
Crouch et al. (2009)
4 more