3g89

X-ray diffraction
1.5Å resolution

T. thermophilus 16S rRNA G527 methyltransferase in complex with AdoMet and AMP in space group P61

Released:

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + guanine(527) in 16S rRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(527) in 16S rRNA
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-192262 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosomal RNA small subunit methyltransferase G Chains: A, B
Molecule details ›
Chains: A, B
Length: 249 amino acids
Theoretical weight: 26.88 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9LCY2 (Residues: 1-249; Coverage: 100%)
Gene names: TTHA1971, gidB, rsmG
Sequence domains: rRNA small subunit methyltransferase G
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments


Cofactor: Ligand SAM 2 x SAM
1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P61
Unit cell:
a: 77.212Å b: 77.212Å c: 167.807Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 0.183 0.21
Expression system: Escherichia coli