› 3g8e

X-ray diffraction
3Å resolution

Crystal Structure of Rattus norvegicus Visfatin/PBEF/Nampt in Complex with an FK866-based inhibitor

Released:
DOI: 10.2210/pdb3g8e/pdb
PDB entry 3g8e coloured by chain, front view.
PDB entry 3g8e coloured by chain, side view.
PDB entry 3g8e coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Crystal structure of Rattus norvegicus Visfatin/PBEF/Nampt in complex with an FK866-based inhibitor.
Kang GB, Bae MH, Kim MK, Im I, Kim YC, Eom SH
Mol Cells 27 667-71 (2009)
PMID: 19533035

Function and Biology Details

Reaction catalysed: 
Nicotinamide D-ribonucleotide + diphosphate = nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182409 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nicotinamide phosphoribosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 491 amino acids
Theoretical weight: 55.51 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q80Z29 (Residues: 1-491; Coverage: 100%)
Gene names: Nampt, Pbef1
Sequence domains:
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
Ligand IS1 2 x IS1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 4A
Spacegroup: P212121
Unit cell:
a: 83.316Å b: 107.406Å c: 120.195Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.295 0.253 0.299
Expression system: Escherichia coli

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Citations

6 review citations

Physiological and pathophysiological roles of NAMPT and NAD metabolism.
Garten et al. (2015)
5 more