› 3g9q

X-ray diffraction
2.6Å resolution

Crystal structure of the FhuD fold-family BSU3320, a periplasmic binding protein component of a Fep/Fec-like ferrichrome ABC transporter from Bacillus subtilis. Northeast Structural Genomics Consortium Target SR577A

Released:
DOI: 10.2210/pdb3g9q/pdb
PDB entry 3g9q coloured by chain, front view.
PDB entry 3g9q coloured by chain, side view.
PDB entry 3g9q coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Entry authors: Forouhar F, Neely H, Seetharaman J, Janjua H, Mao L, Xiao R, Ciccosanti C, Foote EL, Lee D, Nair R, Acton TB, Rost B, Montelione GT, Tong L, Hunt JF, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153440 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Iron(3+)-hydroxamate-binding protein FhuD Chain: A
Molecule details ›
Chain: A
Length: 279 amino acids
Theoretical weight: 31.24 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli
UniProt:
  • Canonical: P37580 (Residues: 45-315; Coverage: 93%)
Gene names: BSU33320, fhuD
Sequence domains: Periplasmic binding protein
Structure domains: Nitrogenase molybdenum iron protein domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P212121
Unit cell:
a: 35.517Å b: 69.898Å c: 114.169Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.222 0.291
Expression system: Escherichia coli

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