› 3g9y

X-ray diffraction
1.4Å resolution

Crystal structure of the second zinc finger from ZRANB2/ZNF265 bound to 6 nt ssRNA sequence AGGUAA

Released:
DOI: 10.2210/pdb3g9y/pdb
PDB entry 3g9y coloured by chain, front view.
PDB entry 3g9y coloured by chain, side view.
PDB entry 3g9y coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The zinc fingers of the SR-like protein ZRANB2 are single-stranded RNA-binding domains that recognize 5' splice site-like sequences.
Loughlin FE, Mansfield RE, Vaz PM, McGrath AP, Setiyaputra S, Gamsjaeger R, Chen ES, Morris BJ, Guss JM, Mackay JP
Proc Natl Acad Sci U S A 106 5581-6 (2009)
PMID: 19304800

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131861 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Zinc finger Ran-binding domain-containing protein 2 Chain: A
Molecule details ›
Chain: A
Length: 33 amino acids
Theoretical weight: 3.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O95218 (Residues: 65-95; Coverage: 9%)
Gene names: ZIS, ZNF265, ZRANB2
Sequence domains: Zn-finger in Ran binding protein and others
RNA (5'-R(*AP*GP*GP*UP*AP*A)-3') Chain: C
Molecule details ›
Chain: C
Length: 6 nucleotides
Theoretical weight: 1.94 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200, APS BEAMLINE 23-ID-D
Spacegroup: P6522
Unit cell:
a: 54.519Å b: 54.519Å c: 48.071Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.203 0.201 0.235
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

4 review citations

Noncoding RNA and Polycomb recruitment.
Brockdorff N. (2013)
3 more

8 mentions without citation

Engineering RNA-binding proteins for biology.
Chen et al. (2013)
7 more