› 3ga2

X-ray diffraction
2.1Å resolution

Crystal structure of the Endonuclease_V (BSU36170) from Bacillus subtilis, Northeast Structural Genomics Consortium Target SR624

Released:
DOI: 10.2210/pdb3ga2/pdb
PDB entry 3ga2 coloured by chain, front view.
PDB entry 3ga2 coloured by chain, side view.
PDB entry 3ga2 coloured by chain, top view.
Source organism: Bacillus subtilis
Entry authors: Forouhar F, Abashidze M, Hussain M, Seetharaman J, Janjua H, Fang Y, Xiao R, Cunningham K, Ma L-C, Owens L, Wang D, Everett JK, Nair R, Acton TB, Rost B, Montelione GT, Tong L, Hunt JF, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage at apurinic or apyrimidinic sites to products with a 5'-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161247 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endonuclease V Chain: A
Molecule details ›
Chain: A
Length: 246 amino acids
Theoretical weight: 28.36 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P96724 (Residues: 1-238; Coverage: 100%)
Gene names: BSU36170, nfi, ywqL
Sequence domains: Endonuclease V
Structure domains: archaeoglobus fulgidus dsm 4304 superfamily

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21212
Unit cell:
a: 61.806Å b: 83.861Å c: 52.287Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.2 0.243
Expression system: Escherichia coli

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