› 3ga5

X-ray diffraction
1.87Å resolution

X-ray structure of glucose/galactose receptor from Salmonella typhimurium in complex with (2R)-glyceryl-beta-D-galactopyranoside

Released:
DOI: 10.2210/pdb3ga5/pdb
PDB entry 3ga5 coloured by chain, front view.
PDB entry 3ga5 coloured by chain, side view.
PDB entry 3ga5 coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Primary publication:
X-ray structure of glucose/galactose receptor from Salmonella typhimurium in complex with the physiological ligand, (2R)-glyceryl-beta-D-galactopyranoside.
Sooriyaarachchi S, Ubhayasekera W, Boos W, Mowbray SL
FEBS J 276 2116-24 (2009)
PMID: 19292879

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150107 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-galactose/methyl-galactoside binding periplasmic protein MglB Chains: A, B
Molecule details ›
Chains: A, B
Length: 309 amino acids
Theoretical weight: 33.46 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Escherichia coli
UniProt:
  • Canonical: P23905 (Residues: 24-332; Coverage: 100%)
Gene names: STM2190, mglB
Sequence domains: Periplasmic binding protein domain
Structure domains: Rossmann fold

Ligands and Environments

4 bound ligands:
Ligand RGG 2 x RGG
Ligand CA 2 x CA
Ligand SCN 1 x SCN
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 36.458Å b: 109.275Å c: 150.703Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.17 0.222
Expression system: Escherichia coli

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Citations

2 citation in other articles

Characterisation of N-linked protein glycosylation in the bacterial pathogen Campylobacter hepaticus.
McDonald et al. (2023)
1 more