› 3gbg

X-ray diffraction
1.9Å resolution

Crystal Structure of ToxT from Vibrio Cholerae O395

Released:
DOI: 10.2210/pdb3gbg/pdb
PDB entry 3gbg coloured by chain, front view.
PDB entry 3gbg coloured by chain, side view.
PDB entry 3gbg coloured by chain, top view.
Source organism: Vibrio cholerae O395
Primary publication:
Structure of Vibrio cholerae ToxT reveals a mechanism for fatty acid regulation of virulence genes.
Lowden MJ, Skorupski K, Pellegrini M, Chiorazzo MG, Taylor RK, Kull FJ
Proc Natl Acad Sci U S A 107 2860-5 (2010)
PMID: 20133655

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107564 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
TCP pilus virulence regulatory protein Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 32.09 KDa
Source organism: Vibrio cholerae O395
Expression system: Escherichia coli
UniProt:
  • Canonical: A5F384 (Residues: 1-276; Coverage: 100%)
Gene names: VC0395_A0363, VC395_0854, tcpN, toxT
Sequence domains: Helix-turn-helix domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PAM 1 x PAM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A, APS BEAMLINE 23-ID-B
Spacegroup: P212121
Unit cell:
a: 39.311Å b: 77.739Å c: 83.568Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.214 0.245
Expression system: Escherichia coli

Quick links

Citations

22 review citations

AraC protein, regulation of the l-arabinose operon in Escherichia coli, and the light switch mechanism of AraC action.
Schleif R. (2010)
21 more

23 mentions without citation

Transcription factor-based biosensors enlightened by the analyte.
Fernandez-López et al. (2015)
22 more