› 3gbm

X-ray diffraction
2.7Å resolution

Crystal Structure of Fab CR6261 in Complex with a H5N1 influenza virus hemagglutinin.

Released:
DOI: 10.2210/pdb3gbm/pdb
PDB entry 3gbm coloured by chain, front view.
PDB entry 3gbm coloured by chain, side view.
PDB entry 3gbm coloured by chain, top view.
Source organisms:
Primary publication:
Antibody recognition of a highly conserved influenza virus epitope.
Ekiert DC, Bhabha G, Elsliger MA, Friesen RH, Jongeneelen M, Throsby M, Goudsmit J, Wilson IA
Science 324 246-51 (2009)
PMID: 19251591

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212355 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (5 distinct):
Hemagglutinin Chains: A, C
Molecule details ›
Chains: A, C
Length: 334 amino acids
Theoretical weight: 37.95 KDa
Source organism: Influenza A virus (A/Viet Nam/1203/2004(H5N1))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q6DQ33 (Residues: 17-346; Coverage: 58%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains:
Hemagglutinin Chains: B, D
Molecule details ›
Chains: B, D
Length: 177 amino acids
Theoretical weight: 20.41 KDa
Source organism: Influenza A virus (A/Viet Nam/1203/2004(H5N1))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q6DQ33 (Residues: 347-520; Coverage: 31%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains: Hemagglutinin Ectodomain; Chain B
antibody (Fab) Chains: H, I
Molecule details ›
Chains: H, I
Length: 226 amino acids
Theoretical weight: 23.98 KDa
Source organism: Homo sapiens
Expression system: Not provided
Structure domains: Immunoglobulins
antibody (Fab) Chains: L, M
Molecule details ›
Chains: L, M
Length: 221 amino acids
Theoretical weight: 23.26 KDa
Source organism: Homo sapiens
Expression system: Not provided
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
4 bound ligands:
Ligand NAG 5 x NAG
Ligand BMA 2 x BMA
Ligand EDO 2 x EDO
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P213
Unit cell:
a: 202.761Å b: 202.761Å c: 202.761Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.203 0.261
Expression systems:
  • Trichoplusia ni
  • Not provided

Quick links

Citations

221 review citations

From vaccines to memory and back.
Sallusto et al. (2010)
220 more

39 mentions without citation

Broadly neutralizing antibodies against influenza viruses.
Laursen et al. (2013)
38 more