› 3gbw

X-ray diffraction
1.32Å resolution

Crystal structure of the first PHR domain of the Mouse Myc-binding protein 2 (MYCBP-2)

Released:
DOI: 10.2210/pdb3gbw/pdb
PDB entry 3gbw coloured by chain, front view.
PDB entry 3gbw coloured by chain, side view.
PDB entry 3gbw coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structures of PHR domains from Mus musculus Phr1 (Mycbp2) explain the loss-of-function mutation (Gly1092-->Glu) of the C. elegans ortholog RPM-1.
Sampathkumar P, Ozyurt SA, Miller SA, Bain KT, Rutter ME, Gheyi T, Abrams B, Wang Y, Atwell S, Luz JG, Thompson DA, Wasserman SR, Emtage JS, Park EC, Rongo C, Jin Y, Klemke RL, Sauder JM, Burley SK
J Mol Biol 397 883-92 (2010)
PMID: 20156452

Function and Biology Details

Reaction catalysed: 
(1a) [E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [RCR-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [RCR-type E3 ubiquitin transferase]-S-ubiquitinyl-L-cysteine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182021 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase MYCBP2 Chain: A
Molecule details ›
Chain: A
Length: 164 amino acids
Theoretical weight: 17.82 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7TPH6 (Residues: 1229-1390; Coverage: 3%)
Gene names: Mycbp2, Pam, Phr1
Sequence domains: PHR domain
Structure domains: PHR domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 2 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P212121
Unit cell:
a: 41.688Å b: 57.767Å c: 64.633Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.162 0.189
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The PHR proteins: intracellular signaling hubs in neuronal development and axon degeneration.
Grill et al. (2016)

4 mentions without citation

Fast and anisotropic flexibility-rigidity index for protein flexibility and fluctuation analysis.
Opron et al. (2014)
3 more