› 3gc2

X-ray diffraction
1.85Å resolution

1.85 Angstrom Crystal Structure of O-succinylbenzoate Synthase from Salmonella typhimurium in Complex with Succinic Acid

Released:
DOI: 10.2210/pdb3gc2/pdb
PDB entry 3gc2 coloured by chain, front view.
PDB entry 3gc2 coloured by chain, side view.
PDB entry 3gc2 coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Entry authors: Minasov G, Wawrzak Z, Skarina T, Onopriyenko O, Peterson SN, Savchenko A, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID), Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
(1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate = 2-succinylbenzoate + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157692 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
o-succinylbenzoate synthase Chain: A
Molecule details ›
Chain: A
Length: 323 amino acids
Theoretical weight: 35.95 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Escherichia coli
UniProt:
  • Canonical: P58486 (Residues: 1-320; Coverage: 100%)
Gene names: STM2306, menC
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand SIN 2 x SIN
Ligand EPE 1 x EPE
Ligand NA 2 x NA
Ligand CL 2 x CL
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P43212
Unit cell:
a: 149.968Å b: 149.968Å c: 39.12Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.155 0.185
Expression system: Escherichia coli

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