› 3gd6

X-ray diffraction
1.6Å resolution

Crystal structure of divergent enolase from Oceanobacillus iheyensis complexed with phosphate

Released:
DOI: 10.2210/pdb3gd6/pdb
PDB entry 3gd6 coloured by chain, front view.
PDB entry 3gd6 coloured by chain, side view.
PDB entry 3gd6 coloured by chain, top view.
Source organism: Oceanobacillus iheyensis HTE831
Entry authors: Fedorov AA, Fedorov EV, Sauder JM, Burley SK, Gerlt JA, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
Galactarate = (2S,3R)-2,3-dihydroxy-5-oxohexanedioate + H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-184425 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Galactarate dehydratase (D-threo-forming) Chain: A
Molecule details ›
Chain: A
Length: 391 amino acids
Theoretical weight: 44.4 KDa
Source organism: Oceanobacillus iheyensis HTE831
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8EMJ9 (Residues: 1-391; Coverage: 100%)
Gene name: OB2843
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: I422
Unit cell:
a: 117.006Å b: 117.006Å c: 134.741Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.236 0.256
Expression system: Escherichia coli

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