› 3gdu

X-ray diffraction
3Å resolution

Crystal structure of DegS H198P/D320A mutant modified by DFP and in complex with YRF peptide

Released:
DOI: 10.2210/pdb3gdu/pdb
PDB entry 3gdu coloured by chain, front view.
PDB entry 3gdu coloured by chain, side view.
PDB entry 3gdu coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
OMP peptides activate the DegS stress-sensor protease by a relief of inhibition mechanism.
Sohn J, Grant RA, Sauer RT
Structure 17 1411-21 (2009)
PMID: 19836340

Function and Biology Details

Reaction catalysed: 
Acts on substrates that are at least partially unfolded. The cleavage site P1 residue is normally between a pair of hydrophobic residues, such as Val-|-Val
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142443 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine endoprotease DegS Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 340 amino acids
Theoretical weight: 36.26 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEE3 (Residues: 27-355; Coverage: 93%)
Gene names: JW3204, b3235, degS, hhoB, htrH
Sequence domains:
Structure domains:
YRF peptide Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 3 amino acids
Theoretical weight: 486 Da
Source organism: Escherichia coli K-12
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MIS 3 x MIS

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2221
Unit cell:
a: 117.572Å b: 171.278Å c: 111.693Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.209 0.231
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

11 review citations

HTRA proteases: regulated proteolysis in protein quality control.
Clausen et al. (2011)
10 more

1 mention without citation

OMP peptides activate the DegS stress-sensor protease by a relief of inhibition mechanism.
Sohn et al. (2009)