› 3gdv

X-ray diffraction
2.49Å resolution

Crystal structure of DegS H198P/D320A mutant modified by DFP and in complex with YQF peptide

Released:
DOI: 10.2210/pdb3gdv/pdb
PDB entry 3gdv coloured by chain, front view.
PDB entry 3gdv coloured by chain, side view.
PDB entry 3gdv coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
OMP peptides activate the DegS stress-sensor protease by a relief of inhibition mechanism.
Sohn J, Grant RA, Sauer RT
Structure 17 1411-21 (2009)
PMID: 19836340

Function and Biology Details

Reaction catalysed: 
Acts on substrates that are at least partially unfolded. The cleavage site P1 residue is normally between a pair of hydrophobic residues, such as Val-|-Val
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142444 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine endoprotease DegS Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 340 amino acids
Theoretical weight: 36.26 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEE3 (Residues: 27-355; Coverage: 93%)
Gene names: JW3204, b3235, degS, hhoB, htrH
Sequence domains:
Structure domains:
YQF peptide Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 3 amino acids
Theoretical weight: 456 Da
Source organism: Escherichia coli K-12
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MIS 3 x MIS

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2221
Unit cell:
a: 118.88Å b: 172.278Å c: 114.768Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.191 0.224
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

11 review citations

HTRA proteases: regulated proteolysis in protein quality control.
Clausen et al. (2011)
10 more

5 mentions without citation

OMP peptides activate the DegS stress-sensor protease by a relief of inhibition mechanism.
Sohn et al. (2009)
4 more