3gdw

X-ray diffraction
2Å resolution

Crystal structure of sigma-54 interaction domain protein from Enterococcus faecalis

Released:
Source organism: Enterococcus faecalis V583
Entry authors: Chang C, Sather A, Buck K, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-182832 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sigma-54 interaction domain protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 139 amino acids
Theoretical weight: 15.9 KDa
Source organism: Enterococcus faecalis V583
Expression system: Escherichia coli
UniProt:
  • Canonical: Q836U6 (Residues: 544-679; Coverage: 15%)
Gene name: EF_1010
Sequence domains: PTS system fructose IIA component
Structure domains: Phosphotransferase system, mannose-type IIA component

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 43.127Å b: 71.418Å c: 94.502Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.183 0.214
Expression system: Escherichia coli