› 3ge1

X-ray diffraction
2.7Å resolution

2.7 Angstrom Crystal Structure of Glycerol Kinase (glpK) from Staphylococcus aureus in Complex with ADP and Glycerol

Released:
DOI: 10.2210/pdb3ge1/pdb
PDB entry 3ge1 coloured by chain, front view.
PDB entry 3ge1 coloured by chain, side view.
PDB entry 3ge1 coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus COL
Entry authors: Minasov G, Brunzelle J, Skarina T, Onopriyenko O, Peterson SN, Savchenko A, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
ATP + glycerol = ADP + sn-glycerol 3-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177195 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycerol kinase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 501 amino acids
Theoretical weight: 56.47 KDa
Source organism: Staphylococcus aureus subsp. aureus COL
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5HGD2 (Residues: 1-498; Coverage: 100%)
Gene names: SACOL1320, glpK
Sequence domains:
Structure domains: Nucleotidyltransferase; domain 5

Ligands and Environments

4 bound ligands:
Ligand ADP 4 x ADP
Ligand GOL 4 x GOL
Ligand PO4 8 x PO4
Ligand CL 4 x CL
1 modified residue:
Ligand MSE 44 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P21
Unit cell:
a: 63.412Å b: 193.651Å c: 91.844Å
α: 90° β: 106.01° γ: 90°
R-values:
R R work R free
0.194 0.191 0.253
Expression system: Escherichia coli

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