› 3gem

X-ray diffraction
1.83Å resolution

Crystal structure of short-chain dehydrogenase from Pseudomonas syringae

Released:
DOI: 10.2210/pdb3gem/pdb
PDB entry 3gem coloured by chain, front view.
PDB entry 3gem coloured by chain, side view.
PDB entry 3gem coloured by chain, top view.
Source organism: Pseudomonas savastanoi pv. phaseolicola 1448A
Entry authors: Osipiuk J, Xu X, Cui H, Nocek B, Savchenko A, Edwards AM, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reactions catalysed: 
5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH
5,6,7,8-tetrahydromonapterin + NADP(+) = 7,8-dihydromonapterin + NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175383 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydromonapterin reductase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 260 amino acids
Theoretical weight: 28.52 KDa
Source organism: Pseudomonas savastanoi pv. phaseolicola 1448A
Expression system: Escherichia coli
UniProt:
  • Canonical: Q48MN0 (Residues: 1-236; Coverage: 100%)
Gene name: PSPPH_1072
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

1 bound ligand:
Ligand ACT 1 x ACT
1 modified residue:
Ligand MSE 24 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 74.198Å b: 73.77Å c: 162.168Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.151 0.149 0.182
Expression system: Escherichia coli

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