› 3gfp

X-ray diffraction
1.8Å resolution

Structure of the C-terminal domain of the DEAD-box protein Dbp5

Released:
DOI: 10.2210/pdb3gfp/pdb
PDB entry 3gfp coloured by chain, front view.
PDB entry 3gfp coloured by chain, side view.
PDB entry 3gfp coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure of the C-terminus of the mRNA export factor Dbp5 reveals the interaction surface for the ATPase activator Gle1.
Dossani ZY, Weirich CS, Erzberger JP, Berger JM, Weis K
Proc Natl Acad Sci U S A 106 16251-6 (2009)
PMID: 19805289

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148976 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent RNA helicase DBP5 Chain: A
Molecule details ›
Chain: A
Length: 189 amino acids
Theoretical weight: 21.37 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P20449 (Residues: 296-482; Coverage: 39%)
Gene names: DBP5, RAT8, YOR046C
Sequence domains: Helicase conserved C-terminal domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P62
Unit cell:
a: 82.423Å b: 82.423Å c: 56.578Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.193 0.191 0.23
Expression system: Escherichia coli

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Citations

9 review citations

The structure of the nuclear pore complex.
Hoelz et al. (2011)
8 more

32 mentions without citation

Live imaging of the cytoskeleton in early cleavage-stage zebrafish embryos.
Wühr et al. (2011)
31 more