› 3gi8

X-ray diffraction
2.59Å resolution

Crystal Structure of ApcT K158A Transporter Bound to 7F11 Monoclonal Fab Fragment

Released:
DOI: 10.2210/pdb3gi8/pdb
PDB entry 3gi8 coloured by chain, front view.
PDB entry 3gi8 coloured by chain, side view.
PDB entry 3gi8 coloured by chain, top view.
Source organisms:
Primary publication:
Structure and mechanism of a Na+-independent amino acid transporter.
Shaffer PL, Goehring A, Shankaranarayanan A, Gouaux E
Science 325 1010-4 (2009)
PMID: 19608859

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212285 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Uncharacterized protein MJ0609 Chain: C
Molecule details ›
Chain: C
Length: 444 amino acids
Theoretical weight: 48.27 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q58026 (Residues: 1-435; Coverage: 100%)
Gene name: MJ0609
Sequence domains: Amino acid permease
Structure domains: Amino acid/polyamine transporter I
7F11 Anti-ApcT Monoclonal Fab Light Chain Chain: L
Molecule details ›
Chain: L
Length: 220 amino acids
Theoretical weight: 24.28 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
7F11 Anti-ApcT Monoclonal Fab Heavy Chain Chain: H
Molecule details ›
Chain: H
Length: 223 amino acids
Theoretical weight: 23.98 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21
Unit cell:
a: 46.039Å b: 111.188Å c: 115.674Å
α: 90° β: 90.92° γ: 90°
R-values:
R R work R free
0.256 0.254 0.307
Expression systems:
  • Escherichia coli
  • Mus musculus

Quick links

Citations

47 review citations

SLC6 neurotransmitter transporters: structure, function, and regulation.
Kristensen et al. (2011)
46 more

10 mentions without citation

Insights into the Structure, Function, and Ligand Discovery of the Large Neutral Amino Acid Transporter 1, LAT1.
Singh et al. (2018)
9 more