› 3gjc

X-ray diffraction
2.8Å resolution

Crystal Structure of the E290S mutant of LeuT with bound OG

Released:
DOI: 10.2210/pdb3gjc/pdb
PDB entry 3gjc coloured by chain, front view.
PDB entry 3gjc coloured by chain, side view.
PDB entry 3gjc coloured by chain, top view.
Source organism: Aquifex aeolicus
Primary publication:
Binding of an octylglucoside detergent molecule in the second substrate (S2) site of LeuT establishes an inhibitor-bound conformation.
Quick M, Winther AM, Shi L, Nissen P, Weinstein H, Javitch JA
Proc Natl Acad Sci U S A 106 5563-8 (2009)
PMID: 19307590

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130578 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Na(+):neurotransmitter symporter (Snf family) Chains: A, B
Molecule details ›
Chains: A, B
Length: 513 amino acids
Theoretical weight: 57.41 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O67854 (Residues: 1-513; Coverage: 100%)
Gene names: aq_2077, snf
Sequence domains: Sodium:neurotransmitter symporter family

Ligands and Environments

3 bound ligands:
Ligand NA 2 x NA
Ligand LEU 2 x LEU
Ligand BOG 8 x BOG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-3
Spacegroup: P21
Unit cell:
a: 83.79Å b: 93.51Å c: 87.46Å
α: 90° β: 94.22° γ: 90°
R-values:
R R work R free
0.223 0.221 0.267
Expression system: Escherichia coli

Quick links

Citations

28 review citations

SLC6 neurotransmitter transporters: structure, function, and regulation.
Kristensen et al. (2011)
27 more

8 mentions without citation

Structure, Function, and Modulation of γ-Aminobutyric Acid Transporter 1 (GAT1) in Neurological Disorders: A Pharmacoinformatic Prospective.
Zafar et al. (2018)
7 more