› 3gkl

X-ray diffraction
2.2Å resolution

Following evolutionary paths to high affinity and selectivity protein-protein interactions using Colicin7 and Immunity proteins

Released:
DOI: 10.2210/pdb3gkl/pdb
PDB entry 3gkl coloured by chain, front view.
PDB entry 3gkl coloured by chain, side view.
PDB entry 3gkl coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Dym O, Tawfik DS, Israel Structural Proteomics Center (ISPC)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146602 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Colicin-E7 Chains: A, B
Molecule details ›
Chains: A, B
Length: 141 amino acids
Theoretical weight: 16.23 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q47112 (Residues: 446-576; Coverage: 23%)
Gene names: cea, colE7
Sequence domains: Colicin/Pyocin-S2, DNase domain
Structure domains: Colicin/pyocin, DNase domain
Colicin-E9 immunity protein Chains: C, D
Molecule details ›
Chains: C, D
Length: 86 amino acids
Theoretical weight: 9.51 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P13479 (Residues: 1-86; Coverage: 100%)
Gene names: ceiE9, imm
Sequence domains: Colicin immunity protein / pyocin immunity protein
Structure domains: Colicin E immunity protein

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 54.161Å b: 67.371Å c: 123.231Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.241 0.24 0.277
Expression system: Escherichia coli

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