› 3gky

X-ray diffraction
1.8Å resolution

The Structural Basis of an ER Stress-Associated Bottleneck in a Protein Folding Landscape

Released:
DOI: 10.2210/pdb3gky/pdb
PDB entry 3gky coloured by chain, front view.
PDB entry 3gky coloured by chain, side view.
PDB entry 3gky coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
Crystal structure of a "nonfoldable" insulin: impaired folding efficiency despite native activity.
Liu M, Wan ZL, Chu YC, Aladdin H, Klaproth B, Choquette M, Hua QX, Mackin RB, Rao JS, De Meyts P, Katsoyannis PG, Arvan P, Weiss MA
J Biol Chem 284 35259-72 (2009)
PMID: 19850922

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134248 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin A chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 21 amino acids
Theoretical weight: 2.41 KDa
Source organism: Sus scrofa
Expression system: Not provided
UniProt:
  • Canonical: P01315 (Residues: 88-108; Coverage: 25%)
Gene name: INS
Insulin B chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 30 amino acids
Theoretical weight: 3.4 KDa
Source organism: Sus scrofa
Expression system: Not provided
UniProt:
  • Canonical: P01315 (Residues: 25-54; Coverage: 36%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand CL 2 x CL
Ligand IPH 1 x IPH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: R3
Unit cell:
a: 78.225Å b: 78.225Å c: 36.712Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.209 0.209 0.253
Expression system: Not provided

Quick links

Citations

8 review citations

Proinsulin misfolding and diabetes: mutant INS gene-induced diabetes of youth.
Liu et al. (2010)
7 more

2 mentions without citation

Structural Lessons From the Mutant Proinsulin Syndrome.
Dhayalan et al. (2021)
1 more