› 3god

X-ray diffraction
2.17Å resolution

Structural basis for DNase activity of a conserved protein implicated in CRISPR-mediated antiviral defense

Released:
DOI: 10.2210/pdb3god/pdb
PDB entry 3god coloured by chain, front view.
PDB entry 3god coloured by chain, side view.
PDB entry 3god coloured by chain, top view.
Source organism: Pseudomonas aeruginosa UCBPP-PA14
Primary publication:
Structural basis for DNase activity of a conserved protein implicated in CRISPR-mediated genome defense.
Wiedenheft B, Zhou K, Jinek M, Coyle SM, Ma W, Doudna JA
Structure 17 904-12 (2009)
PMID: 19523907

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169795 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CRISPR-associated endonuclease Cas1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 328 amino acids
Theoretical weight: 36.69 KDa
Source organism: Pseudomonas aeruginosa UCBPP-PA14
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02ML7 (Residues: 1-324; Coverage: 100%)
Gene names: PA14_33350, cas1
Sequence domains: CRISPR associated protein Cas1
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MN 4 x MN
Ligand NA 2 x NA
Ligand CA 2 x CA
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P212121
Unit cell:
a: 109.87Å b: 110.96Å c: 130.25Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.203 0.258
Expression system: Escherichia coli

Quick links

Citations

55 review citations

CRISPR/Cas, the immune system of bacteria and archaea.
Horvath et al. (2010)
54 more

15 mentions without citation

Evolution and classification of the CRISPR-Cas systems.
Makarova et al. (2011)
14 more