› 3gor

X-ray diffraction
2.51Å resolution

Crystal structure of putative metal-dependent hydrolase APC36150

Released:
DOI: 10.2210/pdb3gor/pdb
PDB entry 3gor coloured by chain, front view.
PDB entry 3gor coloured by chain, side view.
PDB entry 3gor coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
The structure of DinB from Geobacillus stearothermophilus: a representative of a unique four-helix-bundle superfamily.
Cooper DR, Grelewska K, Kim CY, Joachimiak A, Derewenda ZS
Acta Crystallogr Sect F Struct Biol Cryst Commun 66 219-24 (2010)
PMID: 20208147

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112205 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative metal-dependent hydrolase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 157 amino acids
Theoretical weight: 18.63 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: D0VX25 (Residues: 4-157; Coverage: 98%)
Sequence domains: DinB family
Structure domains: dinb family like domain

Ligands and Environments

1 bound ligand:
Ligand NI 4 x NI
1 modified residue:
Ligand MSE 32 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 68.477Å b: 71.47Å c: 123.256Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.186 0.247
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Mycothiol: a target for potentiation of rifampin and other antibiotics against Mycobacterium tuberculosis.
Hernick M. (2013)
4 other articles

3 mentions without citation

The DinB superfamily includes novel mycothiol, bacillithiol, and glutathione S-transferases.
Newton et al. (2011)
2 more