› 3gpm

X-ray diffraction
3.8Å resolution

Structure of the trimeric form of the E113G PCNA mutant protein

Released:
DOI: 10.2210/pdb3gpm/pdb
Model geometry
Fit model/data
PDB entry 3gpm coloured by chain, front view.
PDB entry 3gpm coloured by chain, side view.
PDB entry 3gpm coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
A charged residue at the subunit interface of PCNA promotes trimer formation by destabilizing alternate subunit interactions.
Freudenthal BD, Gakhar L, Ramaswamy S, Washington MT
Acta Crystallogr D Biol Crystallogr 65 560-6 (2009)
PMID: 19465770

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147511 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proliferating cell nuclear antigen Chain: A
Molecule details ›
Chain: A
Length: 258 amino acids
Theoretical weight: 28.87 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P15873 (Residues: 1-258; Coverage: 100%)
Gene names: POL30, YBR0811, YBR088C
Sequence domains:
Structure domains: Proliferating Cell Nuclear Antigen

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P213
Unit cell:
a: 122.093Å b: 122.093Å c: 122.093Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.275 0.273 0.312
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The Many Roles of PCNA in Eukaryotic DNA Replication.
Boehm et al. (2016)
4 other articles

4 mentions without citation

PCNA structure and function: insights from structures of PCNA complexes and post-translationally modified PCNA.
Dieckman et al. (2012)
3 more