› 3gra

X-ray diffraction
2.3Å resolution

Crystal structure of AraC family transcriptional regulator from Pseudomonas putida

Released:
DOI: 10.2210/pdb3gra/pdb
PDB entry 3gra coloured by chain, front view.
PDB entry 3gra coloured by chain, side view.
PDB entry 3gra coloured by chain, top view.
Source organism: Pseudomonas putida KT2440
Entry authors: Bagaria A, Kumaran D, Burley SK, Swaminathan S, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183467 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH araC/xylS-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 202 amino acids
Theoretical weight: 22.3 KDa
Source organism: Pseudomonas putida KT2440
Expression system: Escherichia coli
UniProt:
  • Canonical: Q88H39 (Residues: 28-218; Coverage: 57%)
Gene name: PP_3526
Sequence domains: DJ-1/PfpI family
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:
Ligand EDO 2 x EDO
Ligand MG 1 x MG
Ligand SO4 1 x SO4
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21212
Unit cell:
a: 49.852Å b: 71.876Å c: 104.518Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.268 0.268 0.288
Expression system: Escherichia coli

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