› 3grg

X-ray diffraction
1.9Å resolution

Crystal structure of the F87M/L110M mutant of human transthyretin at pH 7.5

Released:
DOI: 10.2210/pdb3grg/pdb
PDB entry 3grg coloured by chain, front view.
PDB entry 3grg coloured by chain, side view.
PDB entry 3grg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Novel Zn2+-binding sites in human transthyretin: implications for amyloidogenesis and retinol-binding protein recognition.
Palmieri Lde C, Lima LM, Freire JB, Bleicher L, Polikarpov I, Almeida FC, Foguel D
J Biol Chem 285 31731-41 (2010)
PMID: 20659897

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136168 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transthyretin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 127 amino acids
Theoretical weight: 13.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P02766 (Residues: 21-147; Coverage: 100%)
Gene names: PALB, TTR
Sequence domains: HIUase/Transthyretin family
Structure domains: Transthyretin/hydroxyisourate hydrolase domain

Ligands and Environments

3 bound ligands:
Ligand ZN 12 x ZN
Ligand ACT 7 x ACT
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE W01B-MX2
Spacegroup: P21
Unit cell:
a: 83.325Å b: 61.925Å c: 47.3Å
α: 90° β: 89.93° γ: 90°
R-values:
R R work R free
0.194 0.191 0.255
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Hair analysis as a biomonitor for toxicology, disease and health status.
Kempson et al. (2011)
3 more

3 mentions without citation

Novel Zn2+-binding sites in human transthyretin: implications for amyloidogenesis and retinol-binding protein recognition.
Palmieri Lde et al. (2010)
2 more