› 3gri

X-ray diffraction
2Å resolution

The Crystal Structure of a Dihydroorotase from Staphylococcus aureus

Released:
DOI: 10.2210/pdb3gri/pdb
PDB entry 3gri coloured by chain, front view.
PDB entry 3gri coloured by chain, side view.
PDB entry 3gri coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus MW2
Entry authors: Brunzelle JS, Wawrzak Z, Skarina T, Onopriyenko O, Savchenko A, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
(S)-dihydroorotate + H(2)O = N-carbamoyl-L-aspartate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159178 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydroorotase Chains: A, B
Molecule details ›
Chains: A, B
Length: 424 amino acids
Theoretical weight: 46.85 KDa
Source organism: Staphylococcus aureus subsp. aureus MW2
Expression system: Escherichia coli
UniProt:
  • Canonical: P65907 (Residues: 1-424; Coverage: 100%)
Gene names: MW1084, pyrC
Sequence domains: Amidohydrolase family
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand CL 3 x CL
Ligand CA 4 x CA
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P1
Unit cell:
a: 50.217Å b: 55.229Å c: 85.647Å
α: 88.33° β: 76.56° γ: 76.97°
R-values:
R R work R free
0.191 0.189 0.237
Expression system: Escherichia coli

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